Seymour - Melbourne
Via Seymour, Tallarook, Broadford, Kilmore East, Wandong, Heathcote Junction, Wallan, Donnybrook, Craigieburn, Broadmeadows, Essendon, North Melbourne, Southern Cross

Shepparton - Seymour - Melbourne
Via Shepparton, Mooroopna, Murchison East, Nagambie, Seymour, Tallarook, Broadford, Kilmore East, Wandong, Heathcote Junction, Wallan, Donnybrook, Craigieburn, Broadmeadows, Essendon, North Melbourne, Southern Cross

Albury / Wodonga - Seymour - Melbourne
Via Albury (NSW), Wodonga, Chiltern, Springhurst, Wangaratta, Benalla, Violet Town, Euroa, Avenel, Seymour, Tallarook, Broadford, Kilmore East, Wandong, Heathcote Junction, Wallan, Donnybrook, Craigieburn, Broadmeadows, Essendon, North Melbourne, Southern Cross

Melbourne to Sydney
Via Southern Cross, Broadmeadows, Seymour, Benalla, Wangaratta, Albury (NSW), Culcairn (NSW), Henty (NSW), The Rock (NSW), Wagga Wagga (NSW), Junee (NSW), Cootamundra(NSW), Harden (NSW), Yass Junction (NSW), Gunning (NSW), Goulburn (NSW), Moss Vale (NSW), Campbelltown (NSW), Sydney (NSW)

Lilydale - Melbourne
Via Lilydale, Mooroolbark, Croydon, Ringwood East, Ringwood, Heatherdale, Mitcham, Nunawading, Blackburn, Laburnum, Box Hill, Mont Albert, Surrey Hills, Chatham, Canterbury, East Camberwell, Camberwell, Auburn, Glenferrie, Hawthorn, Burnley, East Richmond, Richmond, Parliament, Melbourne Central, Flagstaff, Southern Cross, Flinders Street