Hurstbridge - Melbourne
Via Hurstbridge, Wattle Glen, Diamond Creek, Eltham, Montmorency, Greensborough, Watsonia, Macleod, Rosanna, Heidelberg, Eaglemont, Ivanhoe, Darebin, Alphington, Fairfield, Dennis, Westgarth, Clifton Hill, Victoria Park, Collingwood, North Richmond, West Richmond, Jolimont, Flinders Street, Southern Cross, Flagstaff, Melbourne Central, Parliament

Alamein - Melbourne
Via Alamein, Ashburton, Burwood, Hartwell, Willison, Riversdale, Camberwell, Auburn, Glenferrie, Hawthorn, Burnley, East Richmond, Richmond, Parliament, Melbourne Central, Flagstaff, Southern Cross, Flinders Street

Belgrave - Melbourne
Via Belgrave, Tecoma, Upwey, Upper Ferntree Gully, Ferntree Gully, Boronia, Bayswater, Heathmont, Ringwood, Heatherdale, Mitcham, Nunawading, Blackburn, Laburnum, Box Hill, Mont Albert, Surrey Hills, Chatham, Canterbury, East Camberwell, Camberwell, Auburn, Glenferrie, Hawthorn, Burnley, East Richmond, Richmond, Parliament, Melbourne Central, Flagstaff, Southern Cross, Flinders Street

Craigieburn - Melbourne
Via Craigieburn, Roxburgh Park, Coolaroo, Broadmeadows, Jacana, Glenroy, Oak Park, Pascoe Vale, Strathmore, Glenbervie, Essendon, Moonee Ponds, Ascot Vale, Newmarket, Kensington, North Melbourne, Flagstaff, Melbourne Central, Parliament, Southern Cross, Flinders Street

Cranbourne - Melbourne
Via Cranbourne, Merinda Park, Lynbrook, Dandenong, Yarraman, Noble Park, Sandown Park, Springvale, Westall, Clayton, Huntingdale, Oakleigh, Hughesdale, Murrumbeena, Carnegie, Caulfield, Malvern, Armadale, Toorak, Hawksburn, South Yarra, Burnley, Richmond, Parliament, Melbourne Central, Flagstaff, Southern Cross, Flinders Street

Frankston - Melbourne
Via Frankston, Kananook, Seaford, Carrum, Bonbeach, Chelsea, Edithvale, Aspendale, Mordialloc, Parkdale, Mentone, Cheltenham, Southland (late 2017), Highett, Moorabbin, Patterson, Bentleigh, McKinnon, Ormond, Glenhuntly, Caulfield, Malvern, Armadale, Toorak, Hawksburn, South Yarra, Burnley, Richmond, Parliament, Melbourne Central, Flagstaff, Southern Cross, Flinders Street

Glen Waverley - Melbourne
Via Glen Waverley, Syndal, Mount Waverley, Jordanville, Holmesglen, East Malvern, Darling, Glen Iris, Gardiner, Tooronga, Kooyong, Heyington, Burnley, East Richmond, Richmond, Parliament, Melbourne Central, Flagstaff, Southern Cross, Flinders Street

Hurstbridge - Melbourne
Via Hurstbridge, Wattle Glen, Diamond Creek, Eltham, Montmorency, Greensborough, Watsonia, Macleod, Rosanna, Heidelberg, Eaglemont, Ivanhoe, Darebin, Alphington, Fairfield, Dennis, Westgarth, Clifton Hill, Victoria Park, Collingwood, North Richmond, West Richmond, Jolimont, Flinders Street, Southern Cross, Flagstaff, Melbourne Central, Parliament

Lilydale - Melbourne
Via Lilydale, Mooroolbark, Croydon, Ringwood East, Ringwood, Heatherdale, Mitcham, Nunawading, Blackburn, Laburnum, Box Hill, Mont Albert, Surrey Hills, Chatham, Canterbury, East Camberwell, Camberwell, Auburn, Glenferrie, Hawthorn, Burnley, East Richmond, Richmond, Parliament, Melbourne Central, Flagstaff, Southern Cross, Flinders Street

Pakenham - Melbourne
Via Pakenham, Cardinia Road, Officer, Beaconsfield, Berwick, Narre Warren, Hallam, Dandenong, Yarraman, Noble Park, Sandown Park, Springvale, Westall, Clayton, Huntingdale, Oakleigh, Hughesdale, Murrumbeena, Carnegie, Caulfield, Malvern, Armadale, Toorak, Hawksburn, South Yarra, Burnley, Richmond, Parliament, Melbourne Central, Flagstaff, Southern Cross, Flinders Street

South Morang - Melbourne
Via South Morang, Epping, Lalor, Thomastown, Keon Park, Ruthven, Reservoir, Regent, Preston, Bell, Thornbury, Croxton, Northcote, Merri, Rushall, Clifton Hill, Victoria Park, Collingwood, North Richmond, West Richmond, Jolimont, Flinders Street, Southern Cross, Flagstaff, Melbourne Central, Parliament

Sunbury - Melbourne
Via Sunbury, Diggers Rest, Watergardens, Keilor Plains, St Albans, Ginifer, Albion, Sunshine, Tottenham, West Footscray, Middle Footscray, Footscray, North Melbourne, Flagstaff, Melbourne Central, Parliament, Southern Cross, Flinders Street

Upfield - Melbourne
Via Upfield, Gowrie, Fawkner, Merlynston, Batman, Coburg, Moreland, Anstey, Brunswick, Jewell, Royal Park, Flemington Bridge, Macaulay, North Melbourne, Flagstaff, Melbourne Central, Parliament, Southern Cross, Flinders Street